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MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression <i>in vivo</i>
Xin Zhang, Sihan Lv, Zhenhuan Luo, Yongfei Hu, Xin Peng, Jie Lv, Shanshan Zhao, Jianqi Feng, Guanjie Huang, Qin-Li Wan, Jun Liu, Hongxin Huang, Bing Luan, Dong Wang, Xiaoyang Zhao, Ying Lin, Qinghua Zhou, Zhen-Ning Zhang, Zhili Rong
Publication Date: 22 March, 2021
Article Pages: 14 Open access contents
Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects
Chengkun Wang, Jason K W Cheng, Qianhe Zhang, Nicholas W Hughes, Qiong Xia, Monte M Winslow, Le Cong
Publication Date: 22 February, 2021
Article Pages: 1 Open access contents
A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing
Teruhiko Suzuki, Eiji Katada, Yuki Mizuoka, Satoko Takagi, Yasuhiro Kazuki, Mitsuo Oshimura, Mayumi Shindo, Takahiko Hara
Publication Date: 27 January, 2021
Article Pages: 1 Open access contents
A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs
András Tálas, Krisztina Huszár, Péter István Kulcsár, Julia K Varga, Éva Varga, Eszter Tóth, Zsombor Welker, Gergely Erdős, Péter Ferenc Pach, Ágnes Welker, Zoltán Györgypál, Gábor E Tusnády, Ervin Welker
Publication Date: 15 January, 2021
Article Pages: 1 Open access contents
Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
Ya-Wen Fu, Xin-Yue Dai, Wen-Tian Wang, Zhi-Xue Yang, Juan-Juan Zhao, Jian-Ping Zhang, Wei Wen, Feng Zhang, Kerby C Oberg, Lei Zhang, Tao Cheng, Xiao-Bing Zhang
Publication Date: 04 January, 2021
Article Pages: 16 Open access contents
Precise and broad scope genome editing based on high-specificity Cas9 nickases
Qian Wang, Jin Liu, Josephine M Janssen, Marie Le Bouteiller, Richard L Frock, Manuel A F V Gonçalves
Publication Date: 04 January, 2021
Article Pages: 25 Open access contents
Deploying MMEJ using MENdel in precision gene editing applications for gene therapy and functional genomics
Gabriel Martínez-Gálvez, Parnal Joshi, Iddo Friedberg, Armando Manduca, Stephen C Ekker
Publication Date: 10 December, 2020
Article Pages: 11 Open access contents
CRISPRidentify: identification of CRISPR arrays using machine learning approach
Alexander Mitrofanov, Omer S Alkhnbashi, Sergey A Shmakov, Kira S Makarova, Eugene V Koonin, Rolf Backofen
Publication Date: 08 December, 2020
Article Pages: 1 Open access contents
A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy
Jichao Wang, Xinyue Sui, Yamei Ding, Yingxin Fu, Xinjun Feng, Min Liu, Youming Zhang, Mo Xian, Guang Zhao
Publication Date: 03 December, 2020
Article Pages: 1 Open access contents
A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth
Andrew D Mathis, Ryan M Otto, Kimberly A Reynolds
Publication Date: 22 November, 2020
Article Pages: 1 Open access contents