Introduction

Maximal oxygen uptake (VO_2max) represents the upper physiological limit of the utilization of oxygen for producing energy during strenuous exercise performed until volitional exhaustion [1, 2]. The VO_2max is widely regarded as the gold standard measure of cardiorespiratory fitness and is typically determined using a cardiopulmonary exercise test (CPET) in clinical, applied physiology, and sport and exercise science settings [1, 3–6]. The VO_2max is often used to diagnose cardiovascular disease [7], predict all-cause mortality [8–10], develop exercise prescriptions [3, 11, 12], and evaluate the efficacy of exercise programmes [13–15]. Consequently, the validity of VO_2max values obtained during CPETs has widespread importance in clinical, sporting, and research-related contexts.

The use of indirect calorimetry for the determination of VO_2max during exercise testing to volitional exhaustion on a treadmill or cycle ergometer has become common during the past few decades [16–18]. This has largely been attributed to the development of fast-responding metabolic gas analyzers allowing the time-efficient acquisition of real-time, breath-by-breath, respiratory gas exchange and flow rate data during CPET [see 19 for a review]. These technological advances have contributed to a transition from the Douglas bag method and time-consuming discontinuous step-incremented protocols to more time-efficient continuous ramp or pseudo-ramp protocols for determining VO_2max [20–25]. Despite the considerable progress in the efficiency by which CPET can be conducted and evaluated, there is still much to be learned about the determination of VO_2max [2, 24–30]. One particularly problematic aspect has been the challenge in identifying a lack of VO_2max attainment due to inappropriate test protocols, premature fatigue, or poor participant motivation and lack of effort [31].

The concept of a VO_2max originated almost 100 years ago with the seminal works of Hill and colleagues [32, 33]. They proposed the existence of an individual upper limit or ‘ceiling’ of VO₂ during maximal exercise, beyond which no further increase in VO₂ occurs despite increasing work rate (WR) and higher metabolic demand. The primary criterion for confirming that a VO_2max has been elicited has historically been based on the occurrence of a VO₂ plateau, commonly defined as a small or no increase in VO₂ despite a continued increase in WR [34]. The landmark study of Taylor et al. [34] was the first to use a formal VO₂ plateau criterion, which was defined as an increase in VO₂ of less than 0.150 L/min (or ≤ 2.1 mL·kg^-1·min^-1, considering an average body mass of 72 kg from 115 male participants) in response to a specific discontinuous step-incremented protocol performed over 3–5 laboratory visits. Subsequent studies have often used the Taylor et al. [34] criterion or alternative thresholds to confirm the attainment of a VO₂ plateau [see 29 for a review]. Since the widespread adoption of continuous short-duration and ramp-based CPET protocols, several studies have reported low incidences of the VO₂ plateau [35–39]. The variability in VO₂ plateau incidence has been attributed to differences in the criteria used for detecting the VO₂ plateau [29, 40], VO₂ sampling intervals [36, 41, 42], exercise modality [43], the warm-up prior to the CPET [44], type of CPET protocol [45–48], and various participant characteristics [49–51].

In the absence of a VO₂ plateau, secondary VO_2max criteria based upon achievement of threshold values for the respiratory exchange ratio (RER), percentage of age-predicted maximal heart rate, post-exercise blood lactate concentration, and ratings of perceived exertion (RPE) have become commonly used to evaluate whether a true VO_2max has been attained [29, 40]. However, this approach has been widely criticized by numerous investigators due to the individual variability in maximal physiological responses for these variables and lack of specificity in identifying individuals who did not continue the CPET to their limit of exercise tolerance. Research has shown that some individuals can satisfy some of the secondary criteria thresholds long before the highest VO₂ value observed in the CPET has been attained [2, 29, 37, 39]. The maximal RER criterion, for example, can be satisfied at VO₂ values 27–39% lower than the highest VO₂ value achieved in the CPET [37, 39]. Like the VO₂ plateau, secondary VO_2max criteria are often dependent on exercise modality, test protocol, and participant characteristics [29].

A review by Midgley et al. [29] suggested a new set of standardized VO_2max criteria should be developed that are independent of exercise modality, test protocol, and participant characteristics, so they can be universally applied. In 2009, Midgley and Carroll [28] provided an early narrative review of an evolving test procedure that showed promise for developing more standardized VO_2max criteria, the so-called ‘verification phase’. The verification phase consists of an appended square wave bout of severe-intensity exercise (e.g. above critical power), or similar multistage exercise bout, performed until the limit of exercise tolerance [28]. It is commonly applied after a short recovery period from a CPET, however, longer recovery periods of up to 24–48 hours also have been used [52]. The verification phase is based on the premise that when the highest VO₂ values in the CPET are consistent with the verification phase (typically within 2–3% in accordance with the test-retest reliability of VO_2max), this provides substantial empirical support that the highest possible VO₂ has been elicited. Poole and Jones [2] recently stated that to confirm the attainment of VO_2max a verification phase should be performed at a higher WR than the last load attained in the CPET (i.e. > WR_peak) in all future studies. Conversely, Iannetta et al. [25] recommended WRs within the upper limit of the severe exercise intensity domain to allow the verification phase to be maintained long enough for VO_2max attainment. According to their recent findings, verification phases performed at 110% of the WR_peak attained during CPETs with increment rates of 25 and 30 W/min resulted in exercise durations that were too short to allow VO₂ to reach the highest VO₂ recorded at the end of the preceding ramp CPETs [25]. Along with exercise intensity and duration, it is also unclear whether other factors affect the utility of the verification phase such as exercise modality, differences in the type and duration of the recovery period between the verification phase and CPET, whether a verification criterion threshold is adopted, and participant characteristics such as sex and cardiorespiratory fitness levels.

Given the considerable uncertainty regarding the application of the verification phase, it is feasible to think that a systematic review and meta-analysis is needed to comprehensively summarize the evidence for improving our understanding of the strengths and weaknesses of the substantial number of different verification procedures that have been utilized and its impact on the attainment of VO_2max. Thus, the aim of the present study was to systematically review and provide a meta-analysis on the application of the verification phase for confirming whether the highest possible VO₂ has been attained during ramp or step-incremented CPETs in apparently healthy adults.

Methods

Study selection

Potential studies were screened for inclusion using three methods: (1) title only; (2) title and abstract; and (3) full-text review. Two investigators independently searched and selected articles, and coauthors subsequently confirmed articles to be included in the analysis. Disagreements were resolved by consensus. Agreement between investigators with respect to inclusion and/or exclusion of potential trials was ratified in 252 randomly selected abstracts by means of Cohen’s kappa (κ = 0.811, P < 0.05). Fig 1 summarizes the screening and selection process.

Fig 1

Flowchart of the systematic review and meta-analysis according to the PRISMA guidelines.

VO_2max: maximal oxygen uptake.

Results

The literature search identified 371 potential articles, with 334 obtained from electronic database searches and 37 from the wider inspection of reference lists and electronic citations of these articles. Eighty studies published between 1980 and 2020 met the eligibility criteria and were included in the systematic review (see Fig 1).

Participants

The total number of participants recruited across all included studies was 1,680 (1,077 men, 473 women, and the sex of 130 participants was not specified). Included studies had a median (interquartile range [IQR]) sample size of 13 [10] participants. Participants were aged between 19 and 68 yr, all apparently healthy, and with a physical activity status ranging from sedentary to highly-trained endurance athletes. Thirty-six studies included only men, two included only women, 41 included both men and women, and one study did not specify the sex of the participants (see Table 1). On average, participants had a BMI within the normal range (mean ± SD [range]: 24.4 ± 2.5 [19.4–32.0] kg/m²) and a moderate level of cardiorespiratory fitness (VO_2max mean ± SD [range]: 46.9 ± 12.1 [23.9–68.6] mL·kg^-1·min^-1).

Table 1

Sample characteristics for studies that incorporated a cardiopulmonary exercise test (CPET) (k = 80).

Study				mean values
	Population	Sex	N	Age	BMI	VO2max
		M/F		Years	kg/m2	mL·kg-1·min-1
Alexander and Mier [54]	Soccer players	M/F	5/6	21.3	22.7	57.7
Arad et al. [55]	Sedentary	M	19	33.4	25.8	30.0
		F	16	26.8	26.6	27.1
Astorino and DeRevere [56]	Recreationally trained	M/F	19/11	26	NS	47.2
		M/F	41/38	23.3	NS	40.5
Astorino and White [57]	Physically active	M	13	23.5	24.3	43.8
		F	17	22.9	22.0	40.7
Astorino et al. [53]	Low CRF	M/F	5/5	25.7	22.7	36.2
	Moderate CRF	M/F	5/5	26.3	24.1	46.4
	High CRF	M/F	9/1	26	23.7	57.9
Astorino et al. [58]	Active adults (HIIT-Baseline)	M/F	3/11	27	22	38.0
	Active adults (HIIT—Week 3)					40.4
	Active adults (Control—Baseline)	M/F	8/6	23	24	40.2
	Active adults (Control—Week 3)					40.5
Astorino et al. [59]	Active adults	M/F	14	27	22.5	38.0
Astorino et al. [60]	Sedentary	M/F	6/9	22.4	24.5	32.7
	Sedentary	M/F	1/8	21.8	22.9	42.1
Beltrami et al. [61]	Runners or cross-country skiers	M/F	23/3	29	23.5	61.3
Beltz et al. [62]	Recreationally trained	M	16	23.6	26.6	47.4
Bisi et al. [63]	Healthy adults	M	11	23.5	22.6	35.0
Chidnok et al. [64]	Active adults	M	7	20	24.8	57.7
Clark et al. [65]	Adults of various fitness levels	M/F	3/12	22	22.0	NS
Colakoglu et al. [66]	Athletes	M	9	24.2	23.0	59.7
Colakoglu et al. [67]	Well-trained athletes	M	9	23.6	23.1	60.2
Colakoglu et al. [68]	Athletes	M	9	23.6	23.1	60.2
Dalleck et al. [69]	Healthy adults	M/F	9/9	59.7	27.8	27.7
Day et al. [35]	Healthy adults	M	38	19–61	NS	NS
Del Giudice et al. [70]	Healthy adults	M	14	21.5	22.8	60.2
Dexheimer et al. [71]	Active adults	M	12	29	31.4	50.6
		F	5	25.6	24.4	43.7
Dicks et al. [72]	Firefighters	M	30	34.5	28.7	41.0
Dogra et al. [73]	Older adults (trained)	F	7	62.7	23.4	37.8
	Older adults (untrained)	F	10	68.8	26.1	24.1
Ducrocq et al. [74]	Recreationally trained	M/F	9/4	21.2	22.5	56.0
Elliott et al. [75]	Cyclists	M	8	40.5	25.2	53.7
Faulkner et al. [76]	Recreationally trained	M	13	25.5	24.5	63.9
Foster et al. [77]	Physically active non-athletes (cycling)	M	16	31.5	24.0	51.7
		F	4	28	21.6
	Competitive runners (treadmill running)	M	12	21.6	22.9	56.3
		F	8	21	20.5
Freeberg et al. [78]	Healthy adults	M/F	17/13	21.7	23.7	49.9
Goodall et al. [79]	Cyclists	M	9	28.1	23.1	61.1
Hanson et al. [80]	Recreationally trained	M/F	8/5	24	24.7	56.2
Hawkins et al. [81]	Distance runners	M/F	36/16	NS	NS	63.3
Hogg et al. [82]	Highly trained	M	14	28	23.2	68.6
Iannetta et al. [25]	Recreationally trained	M/F	6/5	28	21.8	52.6
James et al. [83]	Squash players	M/F	6/2	20.3	22.1	48.8
Jamnick et al. [84]	Trained cyclists	M	17	36.2	24.1	62.1
Jamnick et al. [85]	Active adults	M	31	29	25.2	48.6
		F	26	27	23.4	39.8
Johnson et al. [86]	Recreationally trained runners and cyclists	M/F	6/5	22	24.1	46.9
Keiller and Gordon [87]	Recreationally trained	M/F	9/2	22.4	24.4	51.6
Kirkeberg et al. [88]	Recreational-trained men	M	12	29	27.5	49.2
Knaier et al. [89]	Athletes	M	10	27.5	23.1	61.1
		F	7	28.4	22.5	54.3
Knaier et al. [90]	High cardiorespiratory fitness	M	8	27.4	22.8	62.8
		F	5	27.6	22.7	55.2
Kramer et al. [91]	Soccer players	M	15	23.1	23.0	50.5
Mann et al. [92]	Runners	M	20	30	24.2	60.2
		F	12	28	21.7	51.9
Mann et al. [93]	Runners	M	8	36	24.1	57.9
		F	2	32	24.9	49.9
Mauger et al. [94]	Well-trained runners	M	14	22.7	23.4	64.4
McGawley [95]	Recreational runners	M/F	5/5	32	NS	59.8
McKay et al. [96]	Healthy adults	M	12	25	NS	44.5
Midgley et al. [39]	Runners	M	10	39.3	23.6	53.6
	Cyclists	M	10	36.0	23.2	57.7
Midgley et al. [97]	Middle- and long-distance runners	M	16	38.7	23.0	57.1
Midgley et al. [98]	Distance runners	M	9	38.2	24.6	55.0
Mier et al. [99]	College athletes	M/F	8/27	20	23.5	55.5
Murias et al. [100]	Younger adults	M	30	25	24.9	49.4
	Older adults	M	31	68	25.8	33.0
Murias et al. [101]	Older adults	F	6	69	27.0	23.9
	Younger adults	F	8	25	23.8	41.2
Murias et al. [102]	Older adults	M	8	68	26.0	28.3
	Younger adults	M	8	23	25.2	48
Nalcakan [103]	Healthy adults	M	15	21.7	25.0	40.3
Niemela et al. [104]	Healthy adults	M	16	25–35	23.3	42.5
Niemeyer et al. [105]	Physically active	M	24	26.2	24.2	49.8
Niemeyer et al. [106]	Recreationally trained	M	46	25.6	24.0	50.8
Nolan et al. [107]	Active adults	M/F	6/6	23	22.7	57.5
Poole et al. [37]	Healthy adults	M	8	27	NS	50.8
Possamai et al. [108]	Recreationally trained cyclists	M	19	23	25.3	48.0
Riboli et al. [109]	Soccer players	M	16	22.5	22.4	59.2
Rossiter et al. [38]	Healthy adults	M	7	26	25.1	51.5
Sabino-Carvalho et al. [110]	Runners	M	14	22.3	21.2	67.0
		F	4	24	20.4	60.1
Scharhag-Rosenberger et al. [111]	Healthy adults	M/F	20/20	24	23.0	50.0
Scheadler and Devor [112]	Experienced runners	NS	13	25	22.5	64.9
Sedgeman et al. [113]	Recreationally trained	M/F	6/7	29	23.9	50.1
Stachenfeld et al. [114]	Healthy adults	M/F	33/18	30.6	NS	49.2
Straub et al. [115]	Trained cyclists	M	12	33	24.8	56.5
		F	4	38	22.1
Strom et al. [116]	Healthy adults	M/F	21/29	30.3	24.0	47.3
Taylor et al. [117]	Runners and triathlon athletes	M	11	28.5	22.6	63.7
		F	8	26.3	21.8	52.3
Tucker et al. [118]	Nonexercise-trained youth	M	17	27	25.6	41.6
Vogiatzis et al. [119]	Cyclists	M	11	38	22.1	62.0
Weatherwax et al. [120]	Sedentary adults	M	5	53.6	32.0	32.3
		F	11	52.2	29.4	24.8
Weatherwax et al. [15]	Sedentary adults (standardized—baseline)	M/F	4/16	51.2	29.6	24.3
	Sedentary adults (standardized—week 4)	M/F	4/16	51.2	29.7	25.0
	Sedentary adults (standardized—week 8)	M/F	4/16	51.2	29.6	26.3
	Sedentary adults (standardized—week 12)	M/F	4/16	51.2	29.6	26.3
	Sedentary adults (individualized—baseline)	M/F	5/14	44.9	27.2	29.5
	Sedentary adults (individualized—week 4)	M/F	5/14	44.9	27.2	31.1
	Sedentary adults (individualized—week 8)	M/F	5/14	44.9	27.1	31.3
	Sedentary adults (individualized—week 12)	M/F	5/14	44.9	27.0	32.8
Weatherwax et al. [121]	Sedentary adults (control—baseline)	M/F	2/6	45.6	25.5	28.4
	Sedentary adults (control—week 12)				25.5	27.7
	Sedentary adults (standardized—baseline)	M/F	4/16	51.2	29.6	24.3
	Sedentary adults (standardized—week 12)				29.6	26.0
	Sedentary adults (individualized—baseline)	M/F	5/14	44.9	27.1	29.5
	Sedentary adults (individualized—week 12)				26.8	32.8
Weatherwax et al. [122]	Elite endurance-trained	M	18	21.9	19.8	62.8
		F	6	20.2	19.4	51.7
Wilhelm et al. [123]	Healthy adults	M	9	25	25.1	41.0
Williams et al. [124]	Healthy adults	M	8	27	NS	43.0
		M	5	23	NS	48.0
Wingo et al. [125]	Healthy adults	M	9	25	22.4	61.2
Yeh et al. [126]	Healthy adults	M/F	14/1	23.3	21.9	48.9

Abbreviations: BMI = body mass index; CRF = cardiorespiratory fitness level; F = female; HIIT = high-intensity interval training; M = male; NS = not stated; VO_2max = maximal oxygen uptake. Note: Whenever possible, authors were contacted to provide unpublished data.

Characteristics of studies regarding the CPET and verification phase protocols to evaluate VO_2max

Table 2 summarizes the characteristics of the CPET and verification phase protocols of the 80 studies included in this systematic review. Forty-three studies (54%) performed the CPET on a cycle ergometer, 35 (44%) on a treadmill, and two studies (3%) used both modalities. Seventy-three studies (91%) used continuous step-incremented or ramp/pseudo-ramp CPET protocols. Three (4%) used only discontinuous step-incremented protocols. Two studies (3%) used both discontinuous and continuous step-incremented protocols and another two studies (3%) applied self-paced protocols. Thirty-three (41%) of the 80 studies included in the review used one or more VO₂ plateau or secondary VO_2max criteria to confirm the attainment of VO_2max. Thirty studies used the VO₂ plateau, 21 used the heart rate plateau or a criterion based on age-predicted maximal heart rate, 18 used the maximal RER attained in the CPET (RER_max), and 8 used the post-CPET blood lactate concentration.

Table 2

Characteristics of the cardiopulmonary exercise test (CPET) and verification phase protocols used in the reviewed studies (k = 80).

Study	VO2 data sampling method	Traditional VO2max criteria adopted	Exercise Modality	CPET Protocol	Recovery Phase Protocol	Verification Phase (VP) Protocol	Verification Criteria Threshold
Alexander and Mier [54]	30-s time average	VO2 plateau of 2.1 mL·kg-1·min-1	TR	CSI	10-min walking	1st min: ↑ WR until matching the final stage of CPET; then ↑ slope to 2.5% and encouraged to running for 2-min	NS
				DisCSI
Arad et al. [55]	20-s rolling mean	VO2 plateau (linear portion of the VO2-WR relationship); RERmax ≥ 1.10; ≥ 95% APMHR	CYC	Ramp	10-min active and 2–3 min passive	100% WRpeak	NS
Astorino and DeRevere [56]	2×15-s	NS	CYC	Ramp	8-min active	105% WRpeak	CPET vs. VP: VO2max difference ≤ 3.0% and 3.3% and HRmax ≤ 4 bpm
					10-min active	110% WRpeak
Astorino and White [57]	15-s time average	NS	CYC	CSI	10-min active	one stage > CPET-Stagefinal	CPET vs. VP: VO2max difference ≤ 3% and HRmax ≤ 4 bpm
Astorino et al. [53]	2×15-s	NS	CYC	Ramp	8-min active	2-min at 40–45% WRpeak and then 105% WRpeak	CPET vs. VP: VO2max difference < 2 mL·kg-1·min-1
Astorino et al. [58]	30-s time average	NS	CYC	Ramp	10-min active	105% WRpeak	NS
Astorino et al. [59]	30-s time average	NS	CYC	CSI	10-min active	105% WRpeak	VO2max identified as the average of CPET and VP values
Astorino et al. [60]	2×15-s	NS	CYC	Ramp	≥ 24h	105%WRpeak reached in the CPET	NS
	30-s time average				1–1.5h	115%WRpeak reached in the CPET
Beltrami et al. [61]	30-s intervals	VO2 plateau (difference between modelled and actual value >50% of the regression slope for the linear portion of the VO2-WR relationship—an average of 1.7 mL·kg-1·min-1)	TR	CSI (control)	15-min active or passive (self-choose: walk, jog or rest)	1st min at 10 km/h (5% slope) and then ↑ 1 km/h > CPET-Speedpeak	CPET vs. VP: VO2max difference ≤ 123 ± 18 mL/min (or 1.7 mL·kg-1·min-1)
				CSI (reverse)
Beltz et al. [62]	2×15-s	NS	TR	SPV	20-min passive	2-min at 30% CPET-WRpeak, 1-min at 40–45% CPET-WRpeak and then until exhaustion at 105% CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 3%
				Ramp
Bisi et al. [63]	25-s moving-average	VO2 plateau (increase < than 3% or 2.1 mL·kg-1·min-1 between 2 steps of increment); RERmax ≥ 1.08 or 1.15; HRmax within 10 bpm of APMHR	CYC	CSI	6-min active	at least 3 min of cycling at 105% of the WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Chidnok et al. [64]	30-s rolling-mean	NS	CYC	Ramp	Different day	See the formula for a proper reporting 3-min of ’all-out’ cycling	NS
Clark et al. [65]	15-s time average	NS	CYC	CSI	3-min active	WRpeak minus 2 stages	NS
Colakoglu et al. [66]	30-s average	VO2 plateau of 150 mL/min; RERmax ≥ 1.10; ≥ 90% APMHR;	CYC	Ramp	Different day	100% WRpeak	NS
Colakoglu et al. [67]	30-s average	VO2 plateau of 150 mL/min; RERmax ≥ 1.10; HRmax within 10 bpm of APMHR; RPE ≥?	CYC	CSI	Different day	100% WRpeak	VO2 plateau of 150 mL/min; RERmax ≥ 1.10; HRmax within 10 bpm of APMHR; RPE ≥?
Colakoglu et al. [68]	30-s average	VO2 plateau of 150 mL/min; RERmax ≥ 1.10; ≥ 90% APMHR; RPE ≥ 19–20	CYC	CSI	Different day	100%, 105%, and 110% WRpeak to attain the highest VO2peak value	VO2 plateau of 150 mL/min; RERmax ≥ 1.10; ≥ 90% APMHR; RPE ≥ 19–20
Dalleck et al. [69]	2×15-s	NS	CYC	CSI	60-min passive	2-min at 50 Watts; then increased 105% WRpeak	CPET vs. VP: VO2max difference ≤ 3% and HRmax ≤ 4 bpm
Day et al. [35]	30-s time average	NS	CYC	CSI	Different day	90% WRpeak reached in the CPET	NS
Del Giudice et al. [70]	30-s time average	NS	TR	CSI	10-min passive	0.8 km/h > CPET-Speedpeak	NS
Dexheimer et al. [71]	2×15-s	NS	TR	Pseudo-ramp protocol	5-10-min active	105% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Dicks et al. [72]	15-s time average	NS	TR	Pseudo-ramp protocol	3-min active	WRpeak minus 2 stages	NS
Dogra et al. [73]	every 20 ms	NS	CYC	Ramp	Different day	85%WRpeak reached in the CPET	NS
Ducrocq et al. [74]	breath-by-breath	NS	TR	CSI	5-min passive	105% WRpeak	NS
Elliott et al. [75]	10-s epochs	NS	CYC	CSI	60-min	110%WRpeak reached in the CPET	NS
Faulkner et al. [76]	20-s time average	VO2 plateau of 2 mL·kg-1·min-1; RERmax ≥ 1.10; RPE ≥ 17; HRmax within 10 bpm of APMHR; Lamax ≥ 8 mmol	TR	CSI	15-min passive	↑ speed over a 30-second period up to a 1 km/h > CPET-Speedpeak	NS
Foster et al. [77]	30-s time average	rate of increase in VO2 during the last min < 50% when compared to the mid portion of the test	CYC	CSI	1-min active	25 Watts > CPET-WRpeak	NS
			TR	CSI	3-min active	1.6 km/h > CPET-Speedpeak or 0.8 km/h if in the non-athlete group
Freeberg et al. [78]	2×15-s	NS	TR	Incline-based protocol	10-min active	110% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Goodall et al. [79]	30-s mean	NS	CYC	CSI	5-min passive	as described by [38]; however, the intensity was not stated (i.e. 95 or 105%WRpeak reached in the CPET)	NS
Hanson et al. [80]	15-breath moving average	VO2 plateau of 2 mL·kg-1·min-1; RERmax ≥ 1.10	TR	CSI	10-min active	one stage > CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 50 mL/min
Hawkins et al. [81]	40-s Douglas bag collection	NS	TR	CSI	Different day	130% WRpeak	NS
Hogg et al. [82]	30-s time average	VO2 plateau (difference between modelled and actual value > 50% of the regression slope for the linear portion of the VO2-WR relationship); RERmax ≥ 1.10; RPE ≥ 17; HRmax within 10 bpm of APMHR	TR	CSI	10-min active (walking around the laboratory and stretching)	↑ speed over a 30-second period up to a speed stage > CPET-Stagefinal	CPET vs. VP: VO2max difference ≤ 3%
				Incline-based SPV		speed halfway between speedpeak from the SPVincline vs. predicted verification-stage speed of the CSI protocol
				Speed-based SPV		speed halfway between speedpeak from the SPVspeed vs. predicted stage speed of the CSI protocol
Ianetta et al. [25]	20-s rolling mean	VO2 plateau (linear portion of the VO2-WR relationship)	CYC	Ramp	10-min	110% WRpeak	CPET vs. VP: VO2max difference ≤ 0.1 L/min
James et al. [83]	10-s average	VO2 plateau of 2 mL·kg-1·min-1	TR	CSI	5-min active	↑ 1% > CPET-Slope	VO2 plateau of 2 mL·kg-1·min-1
Jamnick et al. [84]	20-s average	NS	CYC	CSI1 (1-min stage length)	5-min passive	90% WRpeak—CSI1	NS
				CSI3 (3-min stage length)
				CSI5 (5-min stage length)
				CSI7 (7-min stage length)
				CSI10 (10-min stage length)
Jamnick et al. [85]	15-s time average	NS	CYC	CSI	3-min active	mean WRpeak minus 2 stages	CPET vs. VP: VO2max difference ≤ 1.5 mL·kg-1·min-1 (or 3% CV)
Johnson et al. [86]	15-s intervals	NS	CYC	CSI	3-min active (50%WRpeak)	WRpeak minus 2 stages	CPET vs. VP: VO2max difference ≤ 3%
Keiller and Gordon [87]	30-s intervals	VO2 plateau (increase < than 50 or 100 mL/min) and HR plateau (increase < than 2 or 4 bpm) over the final two consecutive 30 s sampling periods	TR	CSI (Trials 1 and 2)	6-min passive	10 (female) and 9 (male) km/h and the ↑ 1% > CPET-Slope	CPET vs. VP: HRmax difference ≤ 2 or ≤ 4 bpm
Kirkeberg et al. [88]	30-s time average	NS	TR	CSI (short-term)	3-min active	CPET-Speedend minus 2 stages, where stages were derived using specific equation	NS
				CSI (middle-term)
				CSI (large-term)
Knaier et al. [89]	30-s time average	RERmax ≥ 1.10; ≥ 95% APMHR; RPE ≥ 19; Lamax ≥ 8 mmol	CYC	CSI	10-min active	2 min at 50% WRpeak, 1 min at 70% WRpeak, and then 1 stage > CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Knaier et al. [90]	30-s time average	RERmax ≥1.05, 1.10 and 1.15; 90, 95 and 100% APMHR; RPE ≥ 19 and = 20; Lamax ≥ 8 and 10 mmol	CYC	CSI	10-min active	2 min at 50% WRpeak, 1 min at 70% WRpeak, and then 1 stage > CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Kramer et al. [91]	30-s intervals	NS	TR	CSI	3-min active	2 stages < CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Mann et al. [92]	15-s	NS	TR	CSI	8-10-min	0.5 km/h > CPET-Speedpeak	NS
Mann et al. [93]	15-s	NS	TR	CSI	8-10-min	0.5 km/h > CPET-Speedpeak	NS
Mauger et al. [94]	5-s time average	VO2 plateau (increase < than 1.8 mL·kg-1·min-1 between 2 steps of increment); RERmax ≥ 1.10; HRmax within 10 bpm of APMHR; RPE ≥ 17; Lamax ≥ 8 mmol	TR	CSI	10-min active	one stage > the last completed stage of the CPET	CPET vs. VP: VO2max difference ≤ 1.8 mL·kg-1·min-1
McGawley [95]	30-s time average	VO2 plateau (increase < than 3% or 2 mL·kg-1·min-1 between 2 steps of increment); RERmax ≥ 1.15; HRmax within 10 bpm of APMHR; Lamax ≥ 8 mmol	TR	CSI	9-min passive	105% at CPET-WRpeak (Trials 1 to 5)	CPET vs. VP: VO2max difference ≤ 3%
McKay et al. [96]	15-s time average	NS	CYC	Ramp	5-min active	105%WRpeak reached in the CPET	NS
Midgley et al. [39]	30-s time average	VO2 plateau (difference between modelled and actual value > 50% of the regression slope for the linear portion of the VO2-WR relationship)	CYC	CSI	10-min passive	2 min at 50% WRpeak, 1 min at 70% WRpeak, and then 1 stage > CPET-WRpeak, 2 min at 50% WRpeak, 1 min at 70% WRpeak, and then 1 stage > CPET-WRpeak	CPET vs. VP: modelled and verification VO2 difference > 50% of the regression slope of the individual VO2-WR relationship; HRmax ≤ 4 bpm
			TR
Midgley et al. [97]	30-s time average	absolute plateau in VO2; RERmax ≥ 1.10; HRmax within 10 bpm of APMHR	TR	CSI	10-min active	0.5 km/h > CPET-Speedpeak	CPET vs. VP: VO2max difference ≤ 2% and HRmax ≤ 2 bpm
Midgley et al. [98]	15 and 30-s time average	NS	TR	CSI 1-min stages	5-min passive	one stage > CPET	NS
				DisCSI 2-min stages
				DisCSI 3-min stages
Mier et al. [99]	30-s	VO2 plateau (2 mL·kg-1·min-1 and ≤ SD of the expected increase); RERmax ≥ 1.05, 1.10 and 1.15; ≥ 85% APMHR and HRmax within 10 bpm of APMHR	TR	CSI	10-min active (walking at slow pace)	intensity gradually increased over 2-min until match CPET-WRpeak; after 1 min, the slope was increased 2.5% to running for 2-min	CPET vs. VP: VO2max difference ≤ 2.2 mL·kg-1·min-1
Murias et al. [100]	20-s average time	NS	CYC	Ramp	5-min active	85% WRpeak	CPET vs. VP: VO2max difference ≤ 2.0 mL·kg-1·min-1
						105% WRpeak
Murias et al. [101]	20-s	NS	CYC	Ramp	5-min active	85%WRpeak reached in the CPET	NS
Murias et al. [102]	20-s	NS	CYC	Ramp	5-min active	85%WRpeak reached in the CPET	NS
Nalcakan [103]	30-s	VO2 plateau; RERmax ≥ 1.20; ≥ 90% APMHR	CYC	CSI	Different day	100% WRpeak	NS
Niemela et al. [104]	every min	VO2 plateau (≤60 mL/min for men and ≤50 mL/min for women); adequacy of a subjective criterion for establishing the end point; RERmax ≥ 1.15; HRmax within 10 bpm of APMHR	CYC	CSI I	Different day	1 or 2 sub peak WRs, then 100% of the highest VO2max reached from two CPET	≤5% difference between the ramp test and VP
				CSI II
Niemeyer et al. [105]	30-s time average	< half of expected increase in VO2 (i.e. <4.5 mL·kg-1·min-1)	CYC	Ramp	10-min active	90% WRpeak	CPET vs. VP: VO2max difference ≤ 5%
Niemeyer et al. [106]	30-s time average	VO2 plateau (difference between modelled and actual value > 50% of the regression slope for the linear portion of the VO2-WR relationship)	CYC	Ramp	Different day	90% WRpeak	CPET vs. VP: VO2max difference ≤ 5%
Nolan et al. [107]	2×15-s	NS	TR	CSI	20-min passive	105% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
						115% WRpeak
					60-min passive	105% WRpeak
						115% WRpeak
Poole et al. [37]	20 s	VO2 plateau of regarding the mL/min; RERmax ≥ 1.10, 1.15; HRmax within 10 bpm of APMHR; Lamax ≥ 8 mmol	CYC	Ramp	Different day	105%WRpeak reached in the CPET	NS
Possamai et al. [108]	30-s intervals	plateau in VO2 and HR (i.e. ≤ 50 mL/min or ≤ 2 bpm) over the final two consecutive 30 s sampling periods; HRmax within 10 bpm of APMHR	CYC	CSI	15-min passive	5-min warm-up at the first stage of the CPET; 3-min of passive recovery; 2-min at 20 Watts; then increased 100% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Riboli et al. [109]	30-s intervals	VO2 plateau of 2.1 mL·kg-1·min-1	TR	CSI with 1 min stages	5-min passive	if the CPET did not show a VO2 plateau, a verification bout was performed as described by [38]; however, the intensity was not stated (i.e. 95 or 105%WRpeak reached in the CPET)	NS
				CSI with 2 min stages
				DisCSI
Rossiter et al. [38]	15-s average	VO2 plateau (linear least squares fitting technique)	CYC	Ramp	5-min active	105%WRpeak reached in the CPET	NS
						95%WRpeak reached in the CPET
Sabino-Carvalho et al. [110]	20-s average	NS	TR	DisCSI	3-min passive (standing on treadmill) and 7-min active (walking at 5 km/h)	2-min at 60% WRpeak and then ↑ 0.5 km/h > CPET-Speedpeak	CPET vs. VP: VO2max difference ≤ 2%
Scharhag-Rosenberger et al. [111]	3×10-s average	VO2 plateau (increase < than one-third of the oxygen requirement of a stage change ~ 150 mL/min); RERmax ≥ 1.10; ± 10 bpm APMHR; Lamax > 8 mmol	TR	DisCSI	10-min passive (VerifDay1)	1 min at 60% CPET-Speedpeak and then continued at 110% (or 115% if necessary, a second VF bout in VerifDay1) CPET-Speedpeak	CPET vs. VP: VO2max difference ≤ 5.5%
					Different day (VerifDay2)
Scheadler and Devor [112]	30-s	NS	TR	CSI	Different day	8% slope/ individualized speed for a WR greater than CPET (mean estimated 10.2% WRpeak)	CPET vs. VP: VO2max difference ≤ 50 mL/min
Sedgeman et al. [113]	15-s time average	VO2 plateau of 2.1 mL·kg-1·min-1 during the last two 15-s average samples	CYC	CSI	3-min active	WRpeak minus 2-stages	CPET vs. VP: VO2max difference ≤ 3%
						105%WRpeak
Stachenfeld et al. [114]	20-s averaging	VO2 plateau of 150 mL/min; RERmax ≥ 1.10, 1.15; ≥ 85% APMHR; Lamax ≥ 8 mmol	CYC	CSI	Different day	115% WRpeak reached in the CPET or 125% if the plateau has not been attained	VO2 plateau of 150 mL/min
Straub et al. [115]	15-s time average	NS	CYC	Ramp	10-min passive	1st min: 60% WRpeak and then 110% WRpeak	NS
Strom et al. [116]	30-s time average	NS	TR	CSI	3-min active (walking pace of 67 m/min)	2 stages < CPET-WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Taylor et al. [117]	15-breath average	NS	TR	CSI	15-min active or passive	1st min at 10 km/h (5% slope) and then ↑ 1 km/h > CPET-Speedpeak	NS
Tucker et al. [118]	2×15-s	NS	CYC	CSI	5–10 min active	100%WRpeak	NS
Vogiatzis et al. [119]	NS	NS	CYC	CSI	20-min passive	110% WRpeak	NS
Weatherwax et al. [120]	2×15-s	NS	TR	Pseudo-ramp protocol	20-min passive	105% WRpeak (Trials 1 and 2)	CPET vs. VP: VO2max difference ≤ 3%
Weatherwax et al. [15]	2×15-s	NS	TR	Pseudo-ramp protocol	20-min passive	105% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Weatherwax et al. [121]	2×15-s	NS	TR	Pseudo-ramp protocol	20-min passive	105% WRpeak	CPET vs. VP: VO2max difference ≤ 3%
Weatherwax et al. [122]	2×15-s	NS	TR	DisCSI	20-min passive	3 min at 4.82 km/h and then ↑ 0.64 km/h > CPET-Speedpeak (males)	CPET vs. VP: VO2max difference ≤ 3%
						3 min at 4.82 km/h and then ↑ 0.48 km/h > CPET-Speedpeak (females)
Wilhelm et al. [123]	10-s moving average	NS	CYC	CSI	5-min passive	105%WRpeak	NS
Williams et al. [124]	20-s	NS	CYC	Ramp	5-min active	105%WRpeak	NS
Wingo et al. [125]	2×30-s	VO2 plateau of 135 mL/min; HR within 5 bpm of that on the control test was obtained	CYC	CSI control	20-min passive	100% WRpeak (if <1-min was completed during the last stage of the CPET) or 25 Watts > CPET-WRpeak (if ≥1-min was completed during the last stage of the CPET)	VO2 plateau of 135 mL/min
				CSI post-15 min
				CSI post-45 min
Yeh et al. [126]	NS	NS	TR	CSI	10-min passive	1 km/h > CPET-Speedpeak or 5% slope every minute until exhaustion	NS

Abbreviations: APMHR = age-predicted maximal heart rate; CPET = cardiopulmonary exercise test; CSI = continuous step-incremented; CV = coefficient of variation; CYC = cycling; DisCSI = discontinuous step-incremented; HR = heart rate; HR_max = maximal heart rate; La_max = maximal blood lactate concentration; NS = not stated; RER_max = maximal respiratory exchange ratio; RPE = rating of perceived exertion; SD = standard deviation; SPV = self-paced maximal oxygen uptake; TR = treadmill; VO₂ = oxygen uptake; VO_2max = maximal oxygen uptake; VP = verification phase; WR = work rate; WR_peak = peak work rate. Note: whenever possible, authors were contacted to provide unpublished data.

In terms of processing respiratory VO₂ data at volitional exhaustion, the most common approach was based on time averages. Thirty-eight studies (48%) reported stationary time averages of 5- to 30-s, whereas 29 (36%) used VO₂ data points at fixed intervals of 15- to 30-s, two studies (3%) used 15-breath averages, two studies (3%) used 10-25-s moving averages, one (1%) used 10-s epochs, two (3%) used 20-s rolling averages, one (1%) used 30-s rolling means, and one study (1%) used Douglas bag collections. Four studies (5%) did not detail which VO₂ data processing method was applied.

Regarding the period between the CPET and verification phase procedure, 34 studies (43%) used a short-term active recovery (e.g. pedaling at light-intensity, walking at a slow pace, or stretching) of 1, 3, 5, 6, 8, 10, or 5–10 min, while 26 studies (33%) employed passive recovery of 5, 6, 9, 10, 15, 20, 60, or 60–90 min. Two studies (3%) employed a combination of passive and active recovery and another (1%) used a self-paced approach where participants were permitted to choose their own WR. Three studies (4%) employed short-term recovery (e.g. 8–10 min) without stating whether it was active or passive. Fifteen studies (19%) carried out the verification phase on a different day to the CPET.

Sixty studies (75%) used square-wave verification phase protocols, while 20 studies (25%) used multistage verification protocols characterized by an initial warm-up stage. Overall, 53 studies (66%) adopted “supra WR_peak” verification phases based upon the WR_peak achieved during the CPET (e.g. one treadmill or cycle ergometer WR stage higher than that completed in the CPET, or 105–130% of the WR_peak achieved in the previous CPET). Seven studies (9%) used only 100% of WR_peak, while two other studies (3%) used both WR_peak and supra WR_peak verification phases. Three studies (4%) examined both sub and supra WR_peak within the same study and one study (1%) used a predicted WR based on the following formula to elicit the participant’s limit of tolerance within 180 s: power output = (finite work capacity ÷ 180 s) + critical power. Fourteen studies (18%) used only sub WR_peak verification phases ranging from 85%-95% WR_peak (typically two stages below the WR_peak achieved during the CPET) (see Table 2).

Forty-two studies (53%) employed cut-off points to analyze differences between the highest VO₂ values obtained during the CPET and verification phase to confirm that VO_2max was likely attained. Criteria for VO_2max verification were frequently based on the intra-subject coefficient of variation acquired from the researchers’ laboratories or from published literature, including a VO₂ difference ≤ 2%, ≤ 3%, ≤ 5.0–5.5%, ≤ 1.5–2.2 mL·kg^-1·min^-1, ≤ 50–150 mL/min, or alternative methods.

Quantitative data synthesis: Differences between the highest VO₂ attained in the CPET and verification phase

Table 3 shows comparisons between the highest VO₂ values elicited in the CPET and verification phase for each study. Fig 2 displays the forest plots of effect sizes and 95% CIs for the highest VO₂ values (54 studies) based on the random effects meta-analysis results. Notably, the mean highest VO₂ values were similar between the CPET and verification phase (mean difference = 0.03 [95% CI = -0.01 to 0.06] L/min, P = 0.15). Pooled data for VO_2max following the CPET and verification phase showed no significant heterogeneity among the studies overall (see Fig 2). Except for one of the included studies judged to have a high risk of bias [68], the meta-analyzed studies were judged to have a low-risk of bias as shown by the funnel plot (Fig 3).

Fig 2

Forest plot of all studies included in the meta-analysis (k = 54) for the highest VO₂ responses attained in the cardiopulmonary exercise test and verification phase using random effects analyses.

Data are reported as mean differences (MD) adjusted for control data (95% CIs).

Fig 3

Funnel plot assessment of publication bias for the studies investigating the highest VO₂ responses attained in the cardiopulmonary exercise test and verification phase.

Table 3

Overall comparisons in the meta-analyzed studies for the highest VO₂ values attained in the cardiopulmonary exercise test (CPET) and verification phase (VP) (k = 54).

Study	Specific Experimental Condition		CPET			VP			% Weight	Mean Difference
			Mean [L/min]	SD [L/min]	Total	Mean [L/min]	SD [L/min]	Total		IV, Random, 95%CI [L/min]
Alexander and Mier [54]	CPET protocol (CSI)		3.79	0.39	11	3.80	0.49	11	1.00%	-0.01 [-0.38, 0.36]
	CPET protocol (DisCSI)		3.94	0.40	11	3.84	0.45	11	1.00%	0.10 [-0.25, 0.46]
Arad et al. [55]	N/A		2.18	0.61	35	2.26	0.65	35	1.40%	-0.08 [-0.38, 0.22]
Astorino and DeRevere [56]	CPET-VP recovery (8 min) VP intensity (105% WRpeak)		3.35	1.01	30	3.32	1.00	30	0.50%	0.03 [-0.48, 0.54]
	CPET-VP recovery (10 min) VP intensity (110% WRpeak)		2.82	0.62	79	2.78	0.59	79	3.70%	0.04 [-0.15, 0.23]
Astorino and White [57]	N/A		3.00	0.45	30	3.00	0.45	30	2.50%	0.00 [-0.23, 0.23]
Astorino et al. [53]	Experimental groups (low CRF)		2.35	0.37	10	2.36	0.33	10	1.40%	-0.01 [-0.32, 0.30]
	Experimental groups (moderate CRF)		3.32	0.58	10	3.28	0.60	10	0.50%	0.04 [-0.48, 0.56]
	Experimental groups (high CRF)		4.38	0.70	10	4.29	0.74	10	0.30%	0.09 [-0.54, 0.72]
Astorino et al. [58]	Training effect (HIIT-Baseline)		2.51	0.62	14	2.50	0.61	14	0.60%	0.01 [-0.45, 0.47]
	Training effect (HIIT—Week 3)		2.66	0.67	14	2.60	0.64	14	0.60%	0.06 [-0.43, 0.55]
	Training effect (Control—Baseline)		2.94	0.72	14	2.87	0.71	14	0.50%	0.07 [-0.46, 0.60]
	Training effect (Control—Week 3)		2.97	0.74	14	2.84	0.69	14	0.50%	0.13 [-0.40, 0.66]
Astorino et al. [59]	N/A		2.55	0.62	14	2.57	0.61	14	0.60%	-0.02 [-0.47, 0.43]
Astorino et al. [60]	CPET-VP recovery (at least 24h)		2.37	0.69	15	2.31	0.75	15	0.50%	0.06 [-0.45, 0.58]
	CPET-VP recovery (60 to 90 min)		2.72	0.65	9	2.73	0.72	9	0.30%	-0.01 [-0.64, 0.62]
Beltrami et al. [61]	Experimental groups (control group)		4.50	0.58	13	4.43	0.46	13	0.80%	0.07 [-0.33, 0.47]
	Experimental groups (reverse group)		4.52	0.36	13	4.54	0.33	13	1.90%	-0.02 [-0.28, 0.24]
Beltz et al. [62]	CPET protocol (SPV)		3.84	0.28	16	3.74	0.50	16	1.70%	0.10 [-0.18, 0.38]
	CPET protocol (Ramp)		3.86	0.28	16	3.77	0.50	16	1.70%	0.09 [-0.19, 0.37]
Bisi et al. [63]	N/A		2.41	0.13	11	2.56	0.36	11	2.60%	-0.15 [-0.38, 0.08]
Chidnok et al. [64]	N/A		4.32	0.61	7	4.32	0.69	7	0.30%	0.00 [-0.68, 0.68]
Colakoglu et al. [68]	N/A		4.11	0.69	9	4.56	0.60	9	0.40%	-0.45 [-1.05, 0.15]
Dalleck et al. [69]	N/A		2.33	0.76	18	2.31	0.76	18	0.50%	0.02 [-0.48, 0.52]
Day et al. [35]	N/A		3.64	0.70	38	3.64	0.70	38	1.30%	0.00 [-0.31, 0.31]
Dicks et al. [72]	N/A		3.84	0.65	28	3.72	0.60	28	1.20%	0.12 [-0.21, 0.45]
Ducrocq et al. [74]	N/A		3.73	0.47	13	3.76	0.45	13	1.10%	-0.03 [-0.39, 0.32]
Elliott et al. [75]	N/A		4.26	0.61	8	4.26	0.70	8	0.30%	0.00 [-0.64, 0.64]
Foster et al. [77]	VP exercise modality (TR)		4.09	0.97	20	4.03	1.16	20	0.30%	0.06 [-0.60, 0.72]
	VP exercise modality (CYC)		3.95	0.75	20	4.06	0.75	20	0.60%	-0.11 [-0.57, 0.35]
Freeberg et al. [78]	N/A		3.49	0.85	30	3.49	0.85	30	0.70%	0.00 [-0.43, 0.43]
Goodall et al. [79]	N/A		4.11	0.56	9	3.82	0.71	9	0.40%	0.29 [-0.30, 0.88]
Hogg et al. [82]	N/A		4.87	0.43	14	4.82	0.48	14	1.20%	0.05 [-0.29, 0.39]
Iannetta et al. [25]	WRpeak 5 W/min	1st VP at 110% WRpeak (25 W/min)	3.35	0.68	11	3.30	0.65	11	0.4%	0.05 [-0.51, 0.61]
		2nd VP at 110% WRpeak (5 W/min)	3.35	0.68	11	3.45	0.68	11	0.4%	-0.10 [-0.67, 0.47]
	WRpeak 10 W/min	1st VP at 110% WRpeak (25 W/min)	3.44	0.67	11	3.33	0.62	11	0.4%	0.11 [-0.43, 0.65]
		2nd VP at 110% WRpeak (10 W/min)	3.44	0.67	11	3.47	0.7	11	0.4%	-0.03 [-0.60, 0.54]
	WRpeak 15 W/min	1st VP at 110% WRpeak (25 W/min)	3.44	0.69	11	3.3	0.68	11	0.4%	0.14 [-0.43, 0.71]
		2nd VP at 110% WRpeak (15 W/min)	3.44	0.69	11	3.39	0.64	11	0.4%	0.05 [-0.51, 0.61]
	WRpeak 25 W/min	1st VP at 110% WRpeak (25 W/min)	3.44	0.74	11	3.28	0.67	11	0.4%	0.16 [-0.43, 0.75]
		2nd VP at 110% WRpeak (25 W/min)	3.44	0.74	11	3.29	0.66	11	0.4%	0.15 [-0.44, 0.74]
	WRpeak 30 W/min	1st VP at 110% WRpeak (25 W/min)	3.44	0.72	11	3.31	0.67	11	0.4%	0.13 [-0.45, 0.71]
		2nd VP at 110% WRpeak (30 W/min)	3.44	0.72	11	3.28	0.65	11	0.4%	0.16 [-0.41, 0.73]
Jamnick et al. [84]	CPET protocol (CSI1: 1-min stage length)		4.72	0.41	17	4.65	0.45	17	1.60%	0.07 [-0.22, 0.36]
	CPET protocol (CSI3: 3-min stage length)		4.62	0.42	17	4.56	0.46	17	1.50%	0.06 [-0.23, 0.36]
	CPET protocol (CSI5: 5-min stage length)		4.55	0.46	17	4.55	0.47	17	1.30%	0.00 [-0.31, 0.31]
	CPET protocol (CSI7: 7-min stage length)		4.44	0.42	17	4.37	0.46	17	1.50%	0.07 [-0.22, 0.36]
	CPET protocol (CSI10: 10-min stage length)		4.35	0.43	17	4.23	0.51	17	1.30%	0.12 [-0.20, 0.43]
Jamnick et al. [85]	N/A		3.24	0.57	57	3.25	0.57	57	3.00%	-0.02 [-0.23, 0.19]
Johnson et al. [86]	N/A		3.31	0.76	11	3.34	0.82	11	0.30%	-0.03 [-0.69, 0.63]
Keiller and Gordon [87]	N/A		3.65	0.71	11	3.50	0.58	11	0.50%	0.15 [-0.39, 0.69]
Kirkeberg et al. [88]	CPET protocol (short-term CSI)		4.43	0.48	12	4.41	0.54	12	0.80%	0.03 [-0.38, 0.43]
	CPET protocol (middle-term CSI)		4.40	0.46	12	4.27	0.40	12	1.00%	0.13 [-0.21, 0.47]
	CPET protocol (large-term CSI)		4.42	0.42	12	4.36	0.45	12	1.00%	0.06 [-0.29, 0.41]
Kramer et al. [91]	N/A		3.45	0.29	15	3.42	0.25	15	3.50%	0.03 [-0.16, 0.22]
Mann et al. [93]	N/A		4.11	0.78	10	4.13	0.85	10	0.30%	-0.02 [-0.74, 0.70]
Mann et al. [92]	N/A		3.80	0.87	32	3.78	0.92	32	0.70%	0.03 [-0.41, 0.46]
Mauger et al. [94]	N/A		4.66	0.55	14	4.65	0.59	14	0.70%	0.01 [-0.42, 0.43]
McGawley [95]	N/A		4.08	0.47	10	4.01	0.46	10	0.80%	0.08 [-0.33, 0.48]
Midgley et al. [39]	VP exercise modality (CYC)		3.86	0.39	10	3.92	0.47	10	0.90%	-0.05 [-0.43, 0.33]
	VP exercise modality (TR)		4.05	0.47	10	3.96	0.38	10	0.90%	0.10 [-0.28, 0.47]
Midgley et al. [98]	CPET protocol (CSI 1-min stages)		4.09	0.54	9	4.07	0.53	9	0.50%	0.03 [-0.47, 0.52]
	CPET protocol (DisCSI 2-min stages)		4.10	0.52	9	4.08	0.52	9	0.60%	0.02 [-0.46, 0.50]
	CPET protocol (DisCSI 3-min stages)		3.98	0.49	9	4.07	0.53	9	0.60%	-0.09 [-0.56, 0.38]
Midgley et al. [97]	N/A		4.03	0.42	16	4.01	0.44	16	1.50%	0.01 [-0.28, 0.31]
Mier et al. [99]	N/A		3.64	0.38	10	3.77	0.38	10	1.20%	-0.13 [-0.46, 0.20]
Murias et al. [100]	VP intensity (younger: 85% WRpeak)		3.73	0.51	8	3.76	0.48	8	0.60%	-0.03 [-0.52, 0.45]
	VP intensity (younger: 105% WRpeak)		3.90	0.65	22	3.89	0.64	22	0.90%	0.02 [-0.36, 0.40]
	VP intensity (older: 85% WRpeak)		2.18	0.55	8	2.18	0.55	8	0.50%	0.00 [-0.54, 0.54]
	VP intensity (older: 105% WRpeak)		2.52	0.54	23	2.57	0.51	23	1.40%	-0.05 [-0.36, 0.25]
Niemela et al. [104]	N/A		3.05	0.55	16	3.05	0.49	16	1.00%	0.00 [-0.36, 0.35]
Niemeyer et al. [105]	N/A		4.06	0.43	24	4.06	0.46	24	2.10%	0.00 [-0.25, 0.24]
Niemeyer et al. [106]	N/A		4.01	0.47	46	3.95	0.51	46	3.30%	0.06 [-0.14, 0.26]
Nolan et al. [107]	CPET-VP recovery (20 min) VP intensity (105% WRpeak)		3.64	0.61	12	3.66	0.58	12	0.60%	-0.02 [-0.50, 0.46]
	CPET-VP recovery (20 min) VP intensity (115% WRpeak)		3.68	0.59	12	3.64	0.61	12	0.60%	0.04 [-0.44, 0.52]
	CPET-VP recovery (60 min) VP intensity (105% WRpeak)		3.60	0.58	12	3.60	0.58	12	0.60%	0.00 [-0.46, 0.46]
	CPET-VP recovery (60 min) VP intensity (115% WRpeak)		3.65	0.54	12	3.58	0.60	12	0.60%	0.07 [-0.38, 0.52]
Poole et al. [37]	N/A		4.03	0.28	7	3.95	0.29	7	1.50%	0.08 [-0.22, 0.38]
Possamai et al. [108]	N/A		3.83	0.41	19	3.72	0.42	19	1.90%	0.11 [-0.15, 0.37]
Rossiter et al. [38]	VP intensity (105%WRpeak)		4.15	0.50	5	4.09	0.45	5	0.40%	0.06 [-0.53, 0.65]
	VP intensity (95%WRpeak)		4.11	0.48	5	4.12	0.53	5	0.30%	-0.01 [-0.64, 0.61]
Sabino-Carvalho et al. [110]	Pre-CPET intervention (IPC)		4.24	0.46	16	4.23	0.40	16	1.50%	0.01 [-0.29, 0.31]
	Pre-CPET intervention (Sham)		4.23	0.48	16	4.23	0.43	16	1.30%	0.01 [-0.31, 0.32]
	Pre-CPET intervention (Control)		4.23	0.38	16	4.15	0.32	16	2.20%	0.08 [-0.17, 0.32]
Scharhag-Rosenberger et al. [111]	CPET-VP recovery (same day after 10 min)		3.82	0.99	34	3.72	0.99	34	0.60%	0.10 [-0.37, 0.57]
	CPET-VP recovery (different day)		3.82	0.99	34	3.75	1.00	34	0.60%	0.07 [-0.40, 0.54]
Sedgeman et al. [113]	VP intensity (WRpeak minus 2-stages)		3.69	0.41	13	3.70	0.49	13	1.10%	-0.01 [-0.36, 0.34]
	VP intensity (105%WRpeak)		3.71	0.51	13	3.64	0.50	13	0.90%	0.07 [-0.31, 0.46]
Straub et al. [115]	N/A		3.86	0.73	16	3.84	0.68	16	0.60%	0.02 [-0.47, 0.51]
Taylor et al. [117]	N/A		4.03	0.53	19	3.83	0.52	19	1.20%	0.21 [-0.13, 0.54]
Weatherwax et al. [120]	N/A		2.29	0.73	16	2.29	0.73	16	0.50%	0.00 [-0.50, 0.51]
Weatherwax et al. [15]	Training effect (standardized—baseline)		2.03	0.62	20	2.03	0.60	20	0.90%	0.00 [-0.38, 0.38]
	Training effect (standardized—week 12)		2.17	0.62	20	2.18	0.63	20	0.90%	-0.01 [-0.40, 0.38]
	Training effect (individualized—baseline)		2.37	0.79	19	2.37	0.77	19	0.50%	0.00 [-0.50, 0.50]
	Training effect (individualized—week 12)		2.63	0.89	19	2.65	0.89	19	0.40%	-0.02 [-0.59, 0.55]
Weatherwax et al. [121]	Training effect (control—baseline)		2.18	0.74	8	2.16	0.73	8	0.30%	0.02 [-0.70, 0.74]
	Training effect (control—week 12)		2.11	0.73	8	2.10	0.69	8	0.30%	0.01 [-0.69, 0.71]
	Training effect (standardized—baseline)		2.03	0.62	20	2.03	0.60	20	0.90%	0.00 [-0.38, 0.38]
	Training effect (standardized—week 12)		2.17	0.62	20	2.18	0.63	20	0.90%	-0.01 [-0.40, 0.38]
	Training effect (individualized—baseline)		2.37	0.79	19	2.37	0.77	19	0.50%	0.00 [-0.50, 0.50]
	Training effect (individualized—week 12)		2.63	0.89	19	2.65	0.89	19	0.40%	-0.02 [-0.59, 0.55]
Weatherwax et al. [122]	Experimental groups (males)		3.98	0.36	18	3.94	0.32	18	2.60%	0.04 [-0.19, 0.26]
	Experimental groups (females)		2.68	0.13	6	2.67	0.10	6	8.00%	0.01 [-0.12, 0.14]

Abbreviations: CI = confidence interval; CPET = cardiopulmonary exercise test; CRF = cardiorespiratory fitness level; CSI = continuous step-incremented; CYC = cycling; DisCSI = discontinuous step-incremented; HIIT = high-intensity interval training; IPC = ischemic preconditioning; N/A = not applicable; TR = treadmill; SD = standard deviation; SPV = self-paced maximal oxygen uptake; VO₂ = oxygen uptake; VP = verification phase; WR_peak = peak work rate; W/min = incremental phase based on watts per minute. Note: whenever possible, authors were contacted to provide unpublished data. %Weight = weight attributed to each study due to its statistical power.

Results of subgroup analyses according to the characteristics of the verification phase protocol are summarized in Fig 4. There were no significant differences between the CPET and verification phase for the highest VO₂ values attained after stratifying studies for verification-phase intensity (mean difference = 0.03 [95% CI = -0.01 to 0.07] L/min, P = 0.11), type of recovery utilized (mean difference = 0.02 [95%CI = -0.02 to 0.07] L/min, P = 0.36), VO_2max verification criterion adoption (mean difference = 0.02 [95% CI = -0.02 to 0.06] L/min, P = 0.29), verification procedure with regards to whether or not it was performed on the same day as the CPET (mean difference = 0.03 [95%CI -0.01 to 0.06] L/min, P = 0.21), or verification-phase duration (i.e. no longer than 80 s, from 81 to 120 s and longer than 120 s) (mean difference = 0.03 [95%CI -0.03 to 0.09] L/min, P = 0.35).

Fig 4

Mean differences (95% CIs) between the highest VO₂ responses in the cardiopulmonary exercise test (CPET) and verification phase according to the verification-phase characteristics for intensity (i.e. sub WR_peak vs. supra WR_peak), recovery (i.e. active vs. passive), adoption of criterion threshold (i.e. yes vs. no), timing (performed on the same day vs. a different day to the CPET), and duration (i.e. no longer than 80 s, from 81 to 120 s and longer than 120 s).

Subgroup analyses regarding sex, cardiorespiratory fitness level, exercise modality, and CPET protocol are summarized in Table 4. The median time to exhaustion was 665 s (IQR, 600 s) for the CPET and 148 s (IQR, 110 s) for the verification phase. Considering all sub-analyses presented in Table 4, there were no significant differences between the CPET and verification phase for VO_2max (P = 0.18 to P = 0.71).

Table 4

Subgroup analyses for the cardiopulmonary exercise test (CPET) and verification phase (VP).

	Time to exhaustion (s)			VO2max (L/min)
	N	CPET Mean ± SD	VP Mean ± SD	N	CPET Mean ± SD	VP Mean ± SD	Effect Size (95% CI)	P-value
Sex
Male	146	734 ± 90	244 ± 43	630	3.95 ± 0.48	3.93 ± 0.50	0.02 (-0.02 to 0.08)	0.25
Female	23	659 ± 119	152 ± 46	68	2.63 ± 0.39	2.58 ± 0.40	0.05 (-0.08 to 0.12)	0.71
Both	677	765 ± 140	146 ± 28	941	3.24 ± 0.67	3.21 ± 0.67	0.03 (-0.04 to 0.08)	0.50
Cardiorespiratory fitness level
Low	170	617 ± 111	150 ± 36	322	2.30 ± 0.65	2.32 ± 0.65	0.02 (-0.07 to 0.11)	0.63
Moderate	362	790 ± 101	200 ± 40	565	3.49 ± 0.61	3.45 ± 0.63	0.04 (-0.02 to 0.11)	0.21
High	346	792 ± 149	161 ± 27	716	3.94 ± 0.55	3.90 ± 0.55	0.04 (-0.02 to 0.08)	0.18
Exercise modality
CYC	477	823 ± 143	155 ± 29	916	3.47 ± 0.59	3.45 ± 0.59	0.02 (-0.03 to 0.07)	0.43
TR	386	688 ± 110	189 ± 34	771	3.59 ± 0.58	3.56 ± 0.58	0.03 (-0.02 to 0.08)	0.22
CPET protocol
DisCSI	92	876 ± 120	156 ± 28	169	3.90 ± 0.52	3.87 ± 0.51	0.03 (-0.05 to 0.11)	0.49
CSI	472	696 ± 105	209 ± 40	924	3.71 ± 0.56	3.69 ± 0.58	0.02 (-0.03 to 0.07)	0.38
Ramp	284	848 ± 171	121 ± 23	578	3.16 ± 0.63	3.13 ± 0.62	0.03 (-0.04 to 0.09)	0.44

Group weighted mean differences in maximal oxygen uptake (VO_2max) according to sex, cardiorespiratory fitness level, exercise testing modality, and CPET protocol.

Abbreviations: CI = confidence interval; CPET = cardiopulmonary exercise test; CSI = continuous step-incremented; CYC = cycling; DisCSI = discontinuous step-incremented; TR = treadmill; SD = standard deviation; VP = verification phase.

Discussion

A growing number of studies have included the verification phase procedure to increase confidence that the highest possible VO₂ has been elicited by apparently healthy adults during a CPET. To the best of our knowledge this is the first systematic review and meta-analysis of these studies, and evidences that 90% of which have been published since 2009. The major findings were: (a) in general, the verification phase protocols elicited similar highest VO₂ values to those obtained in the preceding CPET protocols; and (b) concordance between the highest VO₂ values in the CPETs and verification phases were not affected by sex, cardiorespiratory fitness level, exercise modality, CPET protocol, or verification phase protocol.

The present systematic review and meta-analysis shows that the highest mean VO₂ values elicited by verification phase bouts were similar to those elicited in continuous ramp or pseudo-ramp CPET protocols in the majority of studies. In fact, the mean absolute difference of 0.03 L/min for the 54 studies included in the meta-analysis represents a relative difference of only 0.85% between the highest VO₂ values attained in the CPET and verification phase. This is within the most commonly adopted measures of test variability of 2–3% [57, 97]. The present findings also provide evidence that the similarity between the highest VO₂ values attained during the CPETs and verification phases are not affected by sex, cardiorespiratory fitness, exercise modality, CPET protocol design, or how the verification phase was performed (see Table 4 and Fig 4). This contrasts with traditional VO_2max criteria, which are test-protocol dependent and vary according to the individual’s physical characteristics [28, 29]. Day et al. [35], for example, observed that participants with lower cardiorespiratory fitness had a lower tendency to exhibit a deceleration in the VO₂ response at the end of a CPET compared to those with higher cardiorespiratory fitness and, therefore, are less likely to exhibit a VO₂ plateau.

Six of the 54 meta-analyzed studies reported significant mean differences between the highest VO₂ values observed in the CPET and verification phase [25, 55, 56, 68, 87, 95]. Astorino and DeRevere [56], for example, observed significantly higher mean VO_2max values by 0.03 and 0.04 L/min during the CPET than in the verification phase for two samples of participants heterogeneous for cardiorespiratory fitness. However, sub-group analyses revealed that while maximal VO₂ in the CPET was higher than that attained in the verification phase for participants with moderate and high cardiorespiratory fitness, the opposite was true for those with lower cardiorespiratory fitness. Similar findings have been reported by Arad et al. [55], indicating that cardiorespiratory fitness level may be a key moderator of the differences between the highest VO₂ values attained in the CPET and verification phase. A plausible explanation is that individuals with low cardiorespiratory fitness are more susceptible to stopping early during the CPET due to fatigue-associated symptoms [29], which would tend to result in lower VO₂ values. In the present meta-analyses, the mean VO_2max in the verification phase was 8% higher than in the CPET in the low cardiorespiratory fitness group, but 12% and 10% higher in the CPET than in the verification phase in the moderate and high cardiorespiratory fitness groups, respectively (see Table 4). The lack of statistical significance, however, highlights the uncertainty regarding the effects of cardiorespiratory fitness on the differences between the highest VO₂ values in the CPET and verification phase.

Regarding verification-phase duration, Keiller and Gordon [87] observed significantly higher VO₂ values during the incremental treadmill CPETs versus the verification phase with a mean duration of approximately 2 min. This is consistent with the findings of McGawley [95] for 10 recreational runners who performed five consecutive treadmill CPET trials, plus an appended verification phase with a mean duration of < 2 min. Iannetta et al. [25] analyzed the VO₂ responses to ramp-incremented cycling CPETs with WR increments of 5, 10, 15, 25, and 30 W/min, each followed by two verification phases performed at different WRs. The verification phase bouts performed at 110% of the WR_peak from ramp protocols with ramp rates of 25 and 30 W/min (i.e. short verification phase bouts of ~ 80 s) yielded VO₂ values significantly lower than those attained in the CPETs. In contrast, the highest VO₂ values attained during verification phase bouts based on slower WR increments of 5, 10, and 15 W/min, which allowed sufficient time for VO_2max attainment (i.e. 162, 122 and 103 s, respectively) were not different to those achieved in the preceding CPETs. Although the aforementioned studies suggest that verification phase duration is a key moderator for the mean differences between the highest VO₂ observed in the CPET and verification phase, our sub-analysis found no difference for verification-phase durations of ≤ 80 s, ranging from 81 to 120 s, and > 120 s (see Fig 4). Notably, however, only three studies reported short durations of 80 s or less [25, 79, 113] and the lack of statistical significance may be due to the paucity of data.

In contrast to the aforementioned studies [25, 87, 95], Colakoglu et al. [68] observed significantly lower VO₂ values in the CPET versus the verification phase in nine cycling and track and field athletes. According to Midgley et al. [97], if the mean highest VO₂ attained in the verification phase is significantly higher than in the CPET, the investigator should consider that the CPET protocol was inadequate in eliciting the highest possible VO₂ response in all or some of the participants. In the study by Colakoglu et al. [68], participants performed a prolonged step-incremented CPET consisting of one 4-min, three 2-min, and then 1-min increments until volitional exhaustion after 1 h of recovery from a submaximal CPET of at least four 5-min stages. It is feasible that the procedures performed before the maximal CPET may have led to poor participant motivation, lack of effort and premature fatigue in the following test. Additionally, the four verification phase bouts at 100%, 105%, 110%, and 115% of the WR_peak attained in the CPET were performed on four different days to the CPET without any preceding maximal exercise. This also may have positively favored the significantly higher mean VO₂ values in the verification phase compared to the CPET and contrasts with the same-day verification phase used by Keiller and Gordon [87], McGawley [95], and Iannetta et al. [25].

An aim of the present systematic review was to suggest best practices for the application of verification phase protocols. The subgroup analyses revealed no systematic bias between the highest VO₂ values observed in the CPET and verification phase according to the verification-phase intensity (i.e. sub WR_peak vs. supra WR_peak), type of recovery between the CPET and verification phase (i.e. active vs. passive), whether a VO_2max criterion threshold was used for the CPET (i.e. yes vs. no), whether the verification phase was performed in the same testing session or on a different day, and the verification-phase duration (see Fig 4). Considering that differences in the verification phase procedure do not appear to influence its effectiveness, a specific verification procedure currently cannot be recommended. However, some caution must be exercised to avoid an inappropriately high verification-phase WR that results in a short test duration and insufficient time for the highest possible VO₂ to be elicited [25], especially in untrained individuals characterized by slow VO₂ kinetics [127]. Midgley et al. [97] stated that this is a plausible rationale for the early recommendations of Thoden [128], that individuals who do not reach 3 min in a supra WR_peak verification phase should undertake a subsequent verification phase at the same WR or one stage lower than verification-phase the last completed WR stage in the CPET. Poole and Jones [2] suggested that researchers should select a WR that is sufficiently higher than the WR_peak attained in the CPET, such as ~110% WR_peak, to give the VO₂ signal for the higher WR the opportunity to emerge from the extant noise. If the subsequent verification phase produces a VO₂ plateau signifying VO_2max, this signal would be lower than expected for the WR based on the previous VO₂-WR slope. Conversely, Iannetta et al. [25] advocated a verification-phase WR lower than the WR_peak attained in the CPET in order to allow VO_2max to be elicited, since WRs above critical power should elicit VO_2max if the time to exhaustion is sufficiently long. Midgley et al. [39] proposed an alternative approach based on a multistage verification phase protocol that combines WRs below and above WR_peak to obtain a protocol that incorporates a supra WR_peak intensity with a relatively prolonged verification-phase duration. This approach has since been adopted in other studies [39, 53, 54, 61, 62, 64, 69, 76, 82, 87, 89, 90, 99, 104, 108, 110, 111, 115, 117, 122]. Notably, the only study to observe a statistically significant influence of verification phase intensity employed a multistage verification phase protocol incorporating 2 min at 50% of WR_peak, increasing to 70% for an additional minute, and then 105 or 115% until volitional exhaustion [107]. Based on their findings, the authors recommended the use of 105% of the WR_peak attained in the CPET rather than 115% WR_peak. The confounding results and various recommended approaches regarding the verification phase intensity indicates that more research is required before an evidence-based recommendation can be made.

Regarding the recovery time between the CPET and verification phase, intervals between 10–20 min have been commonly used, although in total a wide range of intervals from 1–3 min [65, 77, 88, 113] to 90 min [41] have been used. The present meta-analysis found no significant effect of recovery time on minimizing the difference between the mean VO₂ elicited in the CPET and verification phase. An alternative method is to perform the verification phase on a separate day, although the additional visit to the laboratory and the day-to-day variability in VO_2max [129] might considerably reduce the utility and robustness of this approach. Scharhag-Rosenberger et al. [111] specifically investigated this issue by comparing a 10-min recovery to a verification phase performed on a separate day. No significant difference was observed between the two verification protocols, even though the time to exhaustion was significantly longer when the verification phase was performed on a separate day (2:06 ± 0:22 min vs. 2:42 ± 0:38 min). These findings suggest no advantage in performing the CPET and verification phase on separate days.

Inadequate data processing may negatively impact the utility of the verification phase procedure. Myers et al. [36] suggested small sampling intervals such as 5 and 10 s result in unacceptable variability in VO₂ data, whereas large intervals such as 60 s may not be sufficiently sensitive to accurately track rapid changes in VO₂ such as those observed in ramp and pseudo-ramp CPET protocols. Midgley et al. [130] observed that the reproducibility of VO_2max during continuous step-incremented treadmill CPETs is not affected by the length of the VO₂ time-average interval between the range of 10 to 60 s, however, the actual VO_2max values were significantly different between time averages. The authors suggested that a 30-s stationary time-average for CPETs provides a good compromise between removing noise while maintaining the underlying trend in the VO₂ data. However, no study to date has addressed the effect of the VO₂ sampling interval on the verification phase.

A final issue to be addressed refers to appropriate criteria to accept that the highest possible VO₂ has been achieved. The most common criterion used in the reviewed studies is that the highest VO₂ observed in the verification phase should not exceed 3% of the highest VO₂ obtained in the CPET. This threshold can be justified by the technical error of measurement and intra-individual biological variation associated with the determination of VO_2max [15, 56, 57, 62, 63, 69, 71, 78, 82, 86, 89–91, 95, 107, 108, 113, 116, 120–122]. The more restrictive value of ≤ 2% [97, 110] and the less restrictive values of ≤ 5–5.5% [104–106, 111] may also be appropriate for single or different-day variability. Further research is required before an appropriate verification-phase threshold can be recommended, which provides a high degree of confidence that the difference between the highest VO₂ values observed in the CPET and verification phase are beyond the technical error of measurement and intra-individual biological variation.

Some limitations of the present review need to be acknowledged. First, the meta-analysis only included 79% of the participants that underwent CPET with verification phase protocols in the 80 studies included in the systematic review. This issue was due to unsuccessful attempts to acquire the required unpublished information from some authors. Second, the meta-analysis was based on comparison of the highest VO₂ responses in the CPET and verification phase averaged across study participants. Noakes [131] criticized this approach, stating that the CPET is performed on individuals and not groups and, therefore, the group average approach does not identify individuals who may not have attained VO_2max. A meta-analysis using individual participant data is therefore required. Finally, the present systematic review and meta-analysis comprised only apparently healthy adults and it is still unclear to what extent the use of the verification phase procedure is applicable to special or clinical populations. A growing number of studies have included special or clinical populations such as obese adults [132, 133], breast and prostate cancer survivors [134], wheelchair athletes [135], individuals with spinal-cord injuries [136], patients with heart failure [137] or cystic fibrosis [138–140], and pediatric populations [141–147], including children with spina bifida in an outpatient condition [148], and adolescents with cystic fibrosis [149].

Conclusions

The present meta-analysis showed that the effect sizes calculated from the highest mean VO₂ in apparently healthy adults were similar between CPETs and verification phases performed on a cycle ergometer or treadmill. Furthermore, mean differences between the highest VO₂ values elicited in the CPETs and verification phases were not affected by participant characteristics, exercise modality, or the CPET and verification protocol design. Our findings indicate that from a practical perspective, different procedures may be applied to establish similar highest mean VO₂ responses during the verification phase as compared to the ramp or continuous step-incremented CPETs. It is worth mentioning, however, that some caution must be exercised concerning the selection of sub or supra WR_peak verification phases, since any exercise above the critical power must be of sufficient duration to allow the achievement of the highest possible VO₂ response in the verification phase. Our data reinforce the notion that a verification phase applied after ramp or continuous step-incremented CPETs may provide additional and unbiased evidence that the highest possible VO₂ has been achieved. On the other hand, the invalidation of the highest VO₂ obtained in CPETs by subsequent verification phases was less likely on a group basis. The mean differences in highest VO₂ responses were typically within the test-retest variability of the experimental protocols employed. Accordingly, our findings support the usefulness of the verification phase to confirm the likely attainment of VO₂ on incremental CPET. However, the necessity or mandatory application of the verification phase, especially constant supra WR_peak verification bouts, in all CPET situations remains open to question.